Animal Cell Electroporation and Electrofusion Protocols: v. 48 (Methods in Molecular Biology) - Softcover

Softcover

ISBN 10: 089603304X ISBN 13: 9780896033047

Publisher: Humana Press Inc., 1995

Synopsis

The ability to introduce macromolecules into animal cells, includ­ ing DNA, RNA, proteins, and other bioactive compounds has facili­ tated a broad range of biological studies, from biochemistry and biophysics to molecular biology, cell biology, and whole animal stud­ ies. Gene transfer technology in particular will continue to play an essential role in studies aimed at improving our understanding of the relationships between the gene structure and function, and it has impor­ tant practical applications in both biotechnology and biomedicine, as evidenced by the current intense interest in gene therapy. Although DNA and other macromolecules may be introduced into cells by a variety of methods, including chemical treatments and microinjection, el- troporation has proven to be simpler to perform, more efficient, and effective with a wider variety of cell types than other techniques. The early and broad success of electric field-mediated DNA transfer soon prompted researchers to investigate electroporation for transferring other types of molecules into cells, including RNA, enzymes, antibodies, and analytic dyes. Animal Cell Electroporation and Electrofusion Protocols begins with three chapters that describe the theoretical and practical aspects of electroporation, including a review of the commercially available instrumentation. These introductory chapters will be of particular inter­ est to those new to electric field technologies and to those developing protocols for as yet untested species or cell types. Nineteen chapters follow that present well-tested protocols for electroporation of proteins and DNA into insect, fish, and mammalian cells.

About this title

Review

"This volume should fulfill its aim, to "take the guesswork out of experimental trials." -Society for General Microbiology Quarterly

"The book is very up-to-date, for instance it cover the most important recent developments in experiments with isolation of tumor suppressor genes in melanoma by cDNA microarray. The book represents an outstanding work, which should not be missed in all laboratories dealing with melanoma as well as in all departments where melanoma patients are diagnosed and treated. Those people who are interested to learn how the completion of the Human Genome Project significantly has been contributing to new progress in cancer management, exampled by human malignant melanoma, will certainly enjoy reading this publication as well." - Neoplasma

Synopsis

This superb collection of well-tested protocols for the electroporation of proteins and DNA into insect, fish, and mammalian cells also includes proven animal cell electrofusion techniques for studies of somatic cell genetics and development, and for generating monoclonal antibodies. It is distinguished by its coverage of important model cell types from many organisms and tissue types and its detailed instructions for the growth and preparation of specific cells to achieve optimum transfection. Its extensive reference lists, citations of alternative transfer methods, advice on pitfalls to avoid, and descriptions of expected results ensure readily reproducible results even for beginners.

Edition details

• Publisher: Humana Press Inc.
• Publication date: 1995
• Language: English
• ISBN 10: 089603304X
• ISBN 13: 9780896033047
• Binding: Paperback
• Number of pages: 390
• Editor: Nickoloff Jac A.