Pulsed-field Gel Electrophoresis: Protocols, Methods and Theories: 12 (Methods in Molecular Biology) - Hardcover

Burmeister, Margit; Ulanovsky, Levy

Hardcover

ISBN 10: 0896032299 ISBN 13: 9780896032293

Publisher: Humana Press Inc., 1992

Synopsis

Burmeister and Ulanovsky's new volume on "Pulsed-Field Gel Electrophoresis" presents step-by-step protocols for all users of this powerful methodology, whether novice or expert. The book features a wide range of PFGE techniques, auxiliary methods, and a diverse array of powerful applications. Each protocol is given thorough treatment by authors who have had extensive hands-on-experience with it. Indeed, many of the contributors have long provided assistance and troubleshooting advice to new users of PFGE during the early stages of its growth. Here they incorporate the benefits of their experience in the extensive Notes sections, which describe common problems and pitfalls and offer sensible solutions for dealing with them. Topics and techniques treated include: FIGE, CHEF, OFAGE, TAFE, FORE, ODPFGE; preparation of DNA suitable for PFGE from: bacteria, protozoa, plants, mammals, and yeasts; preparation and analysis of yeast artificial chromosomes (YACS); mapping strategies; biological applications; and, theories and observations. In keeping with the well-known practicality of Humana's "Methods in Molecular Biology" series, "Pulsed-Field Gel Electrophoresis" brims with protocols designed to work even the first time they are used.

About this title

Review

...an excellent reference and experimental manual...a must for every biochemistry and molecular biology library.-Journal of the American Chemical Society

Edition details

• Publisher: Humana Press Inc.
• Publication date: 1992
• Language: English
• ISBN 10: 0896032299
• ISBN 13: 9780896032293
• Binding: Hardcover
• Number of pages: 494