Lab Ref Volume 2: A Handbook of Recipes, Reagents, and Other Reference Tools for Use at the Bench - Softcover
Synopsis
Description Like its predecessor Lab Ref, Volume 1, this book is a handy benchtop source of recipes and information needed for common laboratory protocols. Lab Ref, Volume 2 contains recipes for procedures as diverse as RNA interference, imaging, proteomics, and quantitative nucleic acid analysis, and is organized by application for quick and easy reference. All recipes were derived from recent manuals published by Cold Spring Harbor Laboratory Press, and each recipe is cross-referenced to its manual of origin. Reference tables and charts are included. To meet the growing need for computational resources in the biology laboratory, Lab Ref, Volume 2 also has an expanded Web site section with sources for biological materials, databases for acquiring and annotating genomic and proteomic data, and links to downloadable software. Contents: Introduction: SECTION 1: QUANTITATIVE NUCLEIC ACID ANALYSIS: Isolation of Total DNA; Isolation of Total RNA; Selection of Poly(A) RNA from Total; Isolation and Amplification of RNA; from Single Cells and Small Amounts of Tissue; Hybridization Analysis; Preparation of Nucleic Acid (Target) for Array Hybridization Analysis; Preparation of Nucleic Acid (Probe) for Array Hybridization Analysis; Other Quantitative Methods; SECTION 2: RNAi: RNAi in C. elegans; RNAi in D. melanogaster; RNAi in Plants; RNAi in Mammalians; RNAi in Mouse Oocytes and Early Embryos; SECTION 3: IMAGING: Preparation of Embryos for Live Cell Imaging; Imaging Fixed Tissue; Preparation of Mice for Imaging; Intracellular Imaging; General Information for Imaging; SECTION 4: PROTEIN ANALYSIS/PROTEOMICS: Basic Methods in Protein Analysis for Proteomics; Extraction of Proteins; Advanced Protocols in Identifying Protein Protein Interactions for Proteomics; SECTION 5: ARABIDOPSIS: Microscopy; Genetic Manipulation; Protein Extraction; Nuclear Extraction; Histochemistry of GUS Fusion; SECTION 6: MURINE: In Vitro Culturing of Embryonic Stem Cells; Genotyping; Immunohistochemistry of Whole-mount Embryos; Other Buffers and Solutions; SECTION 7: STORAGE AND SHIPMENT OF BIOLOGICAL SAMPLES: SECTION 8: USEFUL FACTS AND TABLES: A. Useful Information and Reference Tables; B. Nomenclature Guide; C. Useful World Wide Web Sites; Appendix; Index. Note from the Publisher: You may be interested in the following title: Labe Ref volume 1
"synopsis" may belong to another edition of this title.
From the Publisher
This title is part of a series of books, published by Cold
Spring Harbor Laboratory Press, aimed at postgraduates and researchers in
the life sciences. The books provide key practical advice and data to make
your life in the lab as easy as possible. The books are:
At the Bench (9780879697082);
At the Helm (9780879695835);
Binding and Kinetics for Molecular Biologists (9780879697365);
Experimental Design for Biologists (9780879697358);
Lab Dynamics (9780879697419);
Lab Math (9780879696344);
Lab Ref (9780879696306);
Lab Ref, Volume 2 (9780879698157)
Synopsis
Like its predecessor "Lab Ref, Volume 1", this book is a handy benchtop source of recipes and information needed for common laboratory protocols. "Lab Ref, Volume 2" contains recipes for procedures as diverse as RNA interference, imaging, proteomics, and quantitative nucleic acid analysis, and is organized by application for quick and easy reference. All recipes were derived from recent manuals published by Cold Spring Harbor Laboratory Press, and each recipe is cross-referenced to its manual of origin. Reference tables and charts are included. To meet the growing need for computational resources in the biology laboratory, "Lab Ref, Volume 2" also has an expanded Web site section with sources for biological materials, databases for acquiring and annotating genomic and proteomic data, and links to downloadable software.
"About this title" may belong to another edition of this title.
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Lab Ref, Volume 2: A Handbook of Recipes, and Other Reference Tools for Use at the Bench
Published by
Cold Spring Harbor Laboratory Press, 2006
ISBN 10: 0879698152
ISBN 13: 9780879698157
Used
Softcover
Seller: PAPER CAVALIER UK, London, United Kingdom
Seller rating 4 out of 5 stars
Condition: very good. Gently used. May include previous owner's signature or bookplate on the front endpaper, sticker on back and/or remainder mark on text block. Seller Inventory # 9780879698157-3