Purifying Proteins for Proteomics: A Laboratory Manual - Softcover

Simpson, Richard J.

Softcover

ISBN 10: 0879696966 ISBN 13: 9780879696962

Publisher: Cold Spring Harbor Laboratory Press,U.S., 2004

Synopsis

This substantial text provides researchers with detailed descriptions of laboratory methods used to prepare proteins for proteomic analysis using chromatography and electrophoresis. Six chapters are also included for the analysis of purified proteins prior to structural proteomics, with topics including analytical ultracentrifugation, measuring conformational stability, protein characterization with mass spectrometry, and analyzing carbohydrate from glycoproteins. Each chapter is clearly organized in outline form and includes discussion, methods, troubleshooting, and protocols. Appendices are provided that detail protein structure, measuring concentration of proteins, concentrating solutions, protein stabilization for storage, one-dimensional polyacrylamide gel electrophoresis, and troubleshooting guides and cautions. The manual will accompany Proteins and proteomics by the same author. Simpson, who authored many of the chapters as well as editing the text, is at the Joint ProteomicS Laboratory (JPSL) of the Ludwig Institute for Cancer Research in Melbourne, Australia. Annotation ©2004 Book News, Inc., Portland, OR (booknews.com)

About this title

Synopsis

This manual complements Simpson's "Proteins and Proteomics" manual, with a comprehensive collection of methods for protein purification from a variety of source preparations. The protocols are accompanied by extensive discussion of potential pitfalls and troubleshooting advice.

Edition details

• Publisher: Cold Spring Harbor Laboratory Press,U.S.
• Publication date: 2004
• Language: English
• ISBN 10: 0879696966
• ISBN 13: 9780879696962
• Binding: Paperback
• Number of pages: 750