Albeit with new experiments and techniques, this laboratory course manual incorporates previous yeast genetics courses at Cold Spring Harbor. Eleven experiments encompass analysis of yeast cell mapping, transformation, and gene replacement. The fourteen techniques and protocols include: "lazy bones" plasmid transformation of yeast colonies, DNA isolations, and random spore analysis. Technical guidance on media, stock preservation, counting cells, and yeast genetic and physical maps are appended. Does not include an index. Annotation c. by Book News, Inc., Portland, Or.
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This edition of the Cold Spring Harbor Laboratory Press's yeast genetics course manual incorporates elements from the 1994 and 1990 publications. It also contains information on additional techniques such as mitotic recombination and random spore analysis, cytoduction, karyogamy, telomeric silencing, transformation techniques, and isolation of genomic DNA.
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