Strategies for Protein Purification and Characterization: A Laboratory Course Manual - Softcover

Marshak, Daniel R.; Kadonaga, James T.; Burgess, Richard R.; Knuth, Mark W.; Brennan, William A.; Sue-Hwa Lin, MD

Softcover

ISBN 10: 0879693851 ISBN 13: 9780879693855

Publisher: Cold Spring Harbor Press, 1995

Synopsis

Investigators who have identified and cloned a gene of interest often want to isolate and characterize the protein product, yet the methods required are no-toriously tricky for the inexperienced. For the past four years, a course has been held at Cold Spring Harbor Laboratory to teach scientists how to execute the major protein techniques by applying them to four distinct, representative types of molecule: a regulatory protein, a DNA-binding protein, a recombinant protein, and a membrane-bound receptor. This course has now been adapted in the form of a laboratory manual that covers a variety of bulk fractionation, electrophoretic, and chromatographic techniques. Step-by-step protocols are accompanied by troubleshooting advice and guidance on generalizing the techniques for other classes and types of protein. The emphasis throughout is on strategies for purification and characterization rather than automated instrumental analysis.

After years of rigorous testing, these techniques are robust and reliable, and are presented here with the clarity and completeness for which Cold Spring Harbor manuals are celebrated. The book is invaluable for specialists in genetics, microbiology, neuroscience, and cell biology who wish to develop expertise in working with proteins.

About this title

Synopsis

Investigators who have identified and cloned a gene of interest often want to isolate and characterize the protein product, yet the methods required are notoriously difficult for the inexperienced. This manual has been adapted from a course held at the Cold Spring Harbour Laboratory in New York, to teach scientists how to execute the major protein techniques by applying them to four distinct, representative types of molecule: a regulatory protein, a DNA-binding protein, a recombinant protein, and a membrane-bound receptor. This manual covers a variety of bulk fractionation, electrophoretic, and chromatographic techniques. Step-by-step protocols are accompanied by troubleshooting advice and guidance on generalizing the techniques for other classes and types of protein. The emphasis throughout is on strategies for purification and characterization rather than automated instrumental analysis. The book should be of use to specialists in genetics, microbiology, neuroscience, and cell biology, who wish to develop expertise in working with proteins.

Edition details

• Publisher: Cold Spring Harbor Press
• Publication date: 1995
• Language: English
• ISBN 10: 0879693851
• ISBN 13: 9780879693855
• Binding: Spiral-bound
• Number of pages: 396