The increased use of biodegradable synthetic or natural scaffolds combined with cells and/or biological molecules in the area of tissue engineering has led to the need for the development of 'best practice' methods to help ensure the creation of safe, high quality products. Standardisation in cell and tissue engineering introduces concepts and current practice in the field and aims to provide awareness of the importance of standardisation in this area while suggesting directions for further investigation. Part one provides an overview of methods for cell and tissue engineering. Part two begins with a chapter exploring the methods and protocols of standardisation in cell and tissue engineering before moving on to highlight issues of quality control in the area.
Key Features: introduces concepts and current practice in the field of cell and tissue engineering; highlights the importance of standardisation in cell and tissue engineering and suggests directions for further investigation; explores methods and protocols of standardisation in cell and tissue engineering and issues of quality control in cell and tissue engineering.
Contents: Part 1 Methods for cell and tissue engineering: Fundamentals of cell and matrix biology for tissue engineering; Three-dimensional collagen biomatrix development and control; Two- and three-dimensional tissue culture bioprocessing methods for soft tissue engineering; Two- and three-dimensional tissue culture methods for hard tissue engineering; Vascularisation of tissue-engineered constructs. Part 2 Standards and protocols in cell and tissue engineering: Standards in cell and tissue engineering; Principles of good laboratory practice (GLP) for in vitro cell culture applications; Quality control in cell and tissue engineering; Standardised chemical analysis and testing of biomaterials; Sterilisation procedures for tissue allografts; Commercial manufacture of cell therapies.
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Dr Vehid Salih is Senior Lecturer in the Department of Biomaterials and Tissue Engineering at Eastman Dental Institute, UCL, UK.
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