PCR Technology: Current Innovations - Softcover

Griffin, Hugh G.; Griffin, Annette

Softcover

ISBN 10: 0849386748 ISBN 13: 9780849386749

Publisher: CRC Press, 1994

Synopsis

A technique used to amplify the number of copies of a specific region of DNA, the polymerase chain reaction (PCR) is at the forefront of the dramatic development of biochemistry. A bestseller in its first edition, this second edition provides the tools for developing innovative approaches to using this leading technology. It includes theoretical considerations, discussions, and a selection of state-of-the-art techniques for mutation studies, clinical diagnosis, and the detection of food-borne pathogens. This edition also discusses the preparation of PCR experiments, includes examples of analytical PCR divided into qualitative and quantitative applications, and explores preparative methods that address DNA generation for further analysis and in vitro evolution.

About this title

Review

"This second edition provides up-to-date information on PCR technology and applications and covers a selection of widely used procedures and approaches for qualitative as well as quantitative queries. It certainly offers easy to follow, step-by-step protocols and explains the parameters governing PCR reaction outcomes . [A] fundamental reference for scientists new to PCR technology and a source of up-to-date applications for those familiar with the method. I highly recommend this book as a useful tool for hospital scientists who may use PCR technology in their work; or for scientific researchers ." - Australian Journal of Medical Science, Nov. 2004, Vol. 25, No. 4

About the Author

Dr. Tania Nolan is an internationally recognized molecular biologist with a reputation for expertise in the field of mRNA quantification using RT-qPCR. She was a significant coauthor of the specialist textbook The A–Z of Quantitative PCR along with numerous other book chapters and peer-reviewed papers. She has presented several plenary lectures and chaired sessions at major international qPCR meetings.

Stephen A. Bustin, BS(Mod) PhD FSB acquired his first qPCR instrument in 1997 and has published numerous peer-reviewed papers that describe and use this technology. He wrote and edited the A–Z of Quantitative PCR, universally acknowledged as the "qPCR Bible," edited The PCR Revolution, and has written a series of Definitive qPCR ebooks. He also led the international consortium that drew up the MIQE guidelines.

Edition details

• Publisher: CRC Press
• Publication date: 1994
• Language: English
• ISBN 10: 0849386748
• ISBN 13: 9780849386749
• Binding: Paperback
• Edition number: 1
• Number of pages: 384
• Editor: Nolan Tania, Bustin Stephen A.