This book describes analysis techniques and results of topics such as physical backgrounds, chemical backgrounds, and principal methods of topo-optical reactions used in ultrastructure research of the ECM; orientation patterns of GAGs and collagen in different tissues/cartilage, cornea, kidney basement membranes, and skin; factors involved in the formation of submicroscopically ordered matrix structure; and cell-matrix interactions, including cytoskeleton-cell-membrane-matrix relationships. A summarization of the advantages and limitations of polarization microscopy compared to electron microscopy in ultracellular research is also included. Cell biologists, histologists, pathologists, and biochemists in connective tissue research will find this book to be an invaluable reference tool.
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In the wake of the electron microscopy era, which provides the means of direct visualization of the ultrastructure, indirect techniques like polarization microscopy have been increasingly neglected despite its potentialities, when combined with relevant histochemical methods which can selectively increase the optical anisotropy of a (spatially orie
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