Synopsis
Methods in Nucleic Acids Research provides extensively referenced overviews of chapter topics, in addition to step-by-step laboratory protocols. Topics include discussions regarding the preparation and assay of antibodies against oligopeptides, RNA footprinting, gel-retardation assays for nucleic acid binding proteins, in vitro transcription and translation assays for studies of eukaryotic gene expression, human genome mapping, forensic analysis of DNA polymorphism, in situ hybridization for the detection of specific RNA, and other methods. Biochemists, molecular biologists, immunologists, cell biologists, and geneticists will find this book invaluable for their research.
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Synopsis
Includes experimental details pertaining to widely used methods of DNA preparation, quantitation, and analysis. In relation to the analysis of gene structure and function, this volume includes the preparation of synthetic DNA, in vitro site-directed mutagenesis, and in vitro methods for the analysis of eukaryotic transcription and translation. A la
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Methods in Nucleic Acids Research
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