Handbook of Molecular and Cellular Methods in Biology and Medicine - Softcover

Kim, Donghern; Kaufman, Peter B.; Cseke, Leland J.; Wu, William

Softcover

ISBN 10: 0849325110 ISBN 13: 9780849325113

Publisher: CRC Press, 1995

Synopsis

Many of the available books in cellular and molecular biology fail to explain the principles and methods described. Handbook of Molecular and Cellular Methods in Biology and Medicine fills in these gaps. Not only do the authors describe the many methods and techniques most commonly used, but they also discuss why you would use each particular method. This handbook covers a wide range of techniques and methods that have been developed and extensively tested. Described in a logical framework of 21 chapters, methods include isolation and purification of DNA, RNA, and proteins; preparation of hybridization probes; electrophoresis and blotting techniques; construction of cDNA and genomic DNA libraries; DNA sequencing; PCR technology; DNA recombination and mutagenesis; transcription in vitro and translation in vitro; gene transfer and analysis of gene expression in transgenic animals and plants; cell and tissue cultures; and microscopy, as well as bioseparation techniques and preparation of monoclonal and polyclonal antibodies. The authors provide descriptive illustrations for each method. Step-by-step instructions allow you to use this book in the lab while performing the various techniques. Each chapter provides the principles behind the method, easy-to-follow instructions, the reagents and materials required, and references for further study. Many chapters also include a troubleshooting guide, cautioning what to avoid and indicating steps that require extra care. Every biologist needs this book on their lab bench!

About this title

Synopsis

Written by two very highly respected authors, this book covers a wide range of current methods including some new extensively tested techniques. Described in a logical framework of 21 chapters, methods include isolation and purification of DNA, RNA and proteins; preparation of hybridisation probes; blotting techniques; construction of cDNA and genomic DNA libraries; DNA sequencing; PCR technology; DNA recombination and mutagenesis; transcription in vitro and translation in vitro; gene transfer and analysis of gene expression in transgenic animals and plants; cell and tissue cultures and microscopy. Each chapter covers the principle (s), underlying methods and detailed step-by-step protocols. FEATURES: Presents state-of-art technology; Contains troubleshooting techniques.

Edition details

• Publisher: CRC Press
• Publication date: 1995
• Language: English
• ISBN 10: 0849325110
• ISBN 13: 9780849325113
• Binding: Paperback
• Edition number: 1
• Number of pages: 496