PCR Technology: Current Innovations, Second Edition - Softcover

Softcover

ISBN 10: 0849311845 ISBN 13: 9780849311840

Publisher: CRC Press, 2003

Synopsis

A technique used to amplify the number of copies of a specific region of DNA, the polymerase chain reaction (PCR) is at the forefront of the dramatic development of biochemistry. This text provides the tools for developing innovative approaches to using this leading technology. It includes theoretical considerations, discussions, and a selection of state-of-the-art techniques for mutation studies, clinical diagnosis, and the detection of food-borne pathogens. This edition also discusses the preparation of PCR experiments, includes examples of analytical PCR divided into qualitative and quantitative applications, and explores preparative methods that address DNA generation for further analysis and in vitro evolution.

About this title

Review

"This second edition provides up-to-date information on PCR technology and applications and covers a selection of widely used procedures and approaches for qualitative as well as quantitative queries. It certainly offers easy to follow, step-by-step protocols and explains the parameters governing PCR reaction outcomes . [A] fundamental reference for scientists new to PCR technology and a source of up-to-date applications for those familiar with the method. I highly recommend this book as a useful tool for hospital scientists who may use PCR technology in their work; or for scientific researchers ." - Australian Journal of Medical Science, Nov. 2004, Vol. 25, No. 4

Synopsis

In the decade since the publication of the bestselling first edition, Polymerase Chain Reaction (PCR) technology has been at the forefront of dramatic developments in biochemistry. Many exciting advances have been made in this specialized field of DNA analysis and a large number of commercial kits and additives are now available. There has never been a better time for a comprehensive resource covering the current range of PCR strategies and uses."PCR Technology: Current Innovations, Second Edition" is a fundamental reference for scientists new to PCR technology and a source of up-to-date applications for those familiar with the method. It provides theoretical considerations, discussions, and a selection of state-of-the-art techniques for mutation studies, clinical diagnosis, and the detection of food-borne pathogens.The book begins with discussions of the preparation of PCR experiments, followed by examples of analytical PCR divided into qualitative and quantitative applications. The final section explores preparative methods addressing DNA generation for further analysis and in vitro evolution.

Featuring detailed presentations of PCR protocols, this volume contains critical information for practitioners in a wide variety of fields, including forensics, molecular biology research, clinical DNA diagnostics, botany, and paleontology. It provides the essential tools for developing innovative approaches in this leading technology of the genomics revolution.

Edition details

• Publisher: CRC Press
• Publication date: 2003
• Language: English
• ISBN 10: 0849311845
• ISBN 13: 9780849311840
• Binding: Paperback
• Edition number: 2
• Number of pages: 416
• Editor: Weissensteiner Thomas, Nolan Tania, Bustin Stephen A., Griffin Hugh G., Griffin Annette