Highlights of this second edition contain more detailed information on the hot start PCR technique. Expanded discussion of the cell-localizing ability of in situ hybridization includes complete consideration of oligoprobes. Other topics featured are: the detection of such viruses as the human papillomavirus and the human immunodeficiency virus-1; RT in situ PCR, including step-by-step protocols and applications; and an update on the detection of hepatitis C and measles. Dr Nuovo is the co-author of "Genital Papillomaviruses and Related Neoplasms".
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