This is the fourth volume of the highly regarded 'Physics of Cancer (Second Edition)' series, written with the aim of making very important topics in the physics of cancer visible to the research community. This fourth volume deals with mechanobiology using biophysical methods. The first chapter deals with mechanosensing and mechanotransduction on vastly different length scales. The second chapter discusses biophysical techniques for mechanical phenotype characterization of cells and nuclei. The third provides an overview of the mechanical phenotype of the plasma membrane. The fourth chapter contains the latest insights into the mechanical assessment of cell spheroids, organoids and tumoroids. The basic approach of this text is to present the latest promising findings to the scientific community, addressing scientists at all career stages.
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