Although longer and containing greater detail, the third edition of this introductory text on gene cloning is still aimed at undergraduates and others who have little or no previous experience of cloning techniques. It includes an entirely new chapter on the polymerase chain reaction, a technique that has assumed great importance in molecular biology. There is also a new final chapter on plant genetic engineering, reflecting the increasing research interest, and general concern, in promoting environmentally-sensitive practices in agriculture.
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Dr Terry Brown is a lecturer in the Department of Biochemistry and Applied Molecular Biology at the University of Manchester Institute of Science and Technology in the UK.
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