Immunochem.practice 2e - Softcover

JOHNSTONE

Softcover

ISBN 10: 0632017236 ISBN 13: 9780632017232

Publisher: Wiley–Blackwell, 1987

Synopsis

The purpose of this practical book is to provide scientists, whatever their specialty, with an account of the various techniques available for detecting, estimating and isolating biological molecules. The methods presented are based on the range of immune reactions that have been discovered in recent years, the usefulness of which has quickly been put to work in many research programmes, as well as the biotechnological industries. At the forefront of the advances made, has been an improved understanding of the role of monoclonal antibodies. Each chapter in the second edition of "Immunochemistry in Practice" has been updated to take these advances into consideration. Clear guidance on purifying, digesting and applying fractions has been given along with a wide coverage of immunohistochemistry and immunoblotting. The sections on the immunoradiometric assays, ELISA and IRMA, have also been expanded in this edition.

About this title

From the Back Cover

This practical book provides scientists of all disciplines with clear descriptions of the plethora of immunochemical techniques available for detecting, isolating and characterising biological molecules. The revolution in molecular biology and biotechnology in the past decade has meant that immunochemistry has become an integral part of the experimental armoury of scientists from a variety of backgrounds. At the heart of this technology is the remarkable specificity that antibodies display toward their epitopes. The specific recognition offered by immunological molecules has resulted in their uptake for a wide range of purposes. This book explains how to produce and purify antibodies and how to tag them for later visualisation.

Edition details

• Publisher: Wiley–Blackwell
• Publication date: 1987
• Language: English
• ISBN 10: 0632017236
• ISBN 13: 9780632017232
• Binding: Paperback
• Number of pages: 318