Updated to reflect advances in the field, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Written for advanced undergraduates, graduates and scientists who want to use this technology, emphasis is placed on the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations. An introduction to the basic biochemical principles is presented first. Then PCR and cloning using E. coli hosts and plasmid, phage and hybrid vectors are described, followed by the generation and screening of libraries and how to modify, inactivate or express cloned sequences. Finally genetic manipulation in a range of other organisms is discussed, including other bacteria, fungi, algae and plants, insects and mammals. A series of 'real-life' biological problems are also presented to enable readers to assess their understanding of the material and to prepare for exams.
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..".will give any new student a foothold in molecular biology....provides a cheap and up-to-date introduction to many fundamental techniques." Jody D. Berry, CSM Newsletter
Book Description:Updated to reflect advances in the field, this introduction provides a broad but concise coverage of recombinant DNA techniques. It is written specifically for students and researchers who want to use this technology, with emphasis placed on understanding the underlying concepts, thereby enabling the reader to devise their own strategies.
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1. Gene Cloning and Manipulation
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Cambridge University Press, Cambridge, UK
(2007)
ISBN 10: 0521817935
ISBN 13: 9780521817936
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Book Description Cambridge University Press, Cambridge, UK, 2007. Hardcover. Condition: New. Dust Jacket Condition: New. Second Edition. Now fully updated to reflect recent advances, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Written for advanced undergraduates, graduates and scientists who want to use this technology, emphasis is placed on the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations. An introduction to the basic biochemical principles is presented first. Then PCR and cloning using E. coli hosts and plasmid, phage and hybrid vectors are described, followed by the generation and screening of libraries and how to modify, inactivate or express cloned sequences. Finally genetic manipulation in a range of other organisms is discussed, including other bacteria, fungi, algae and plants, insects and mammals. A series of 'real-life' biological problems are also presented to enable readers to assess their understanding of the material and to prepare for exams. Covers a broad range of host organisms, from E. coli to mammals Focuses on the underlying principles thereby equipping the reader to devise appropriate strategies for novel situations and for use in their own research Contains example scenarios, which allow the reader to test their understanding of the material and apply it to real-life research situations Contents Preface; 1. The tools for the job; 2. Polymerase chain reaction; 3. Simple cloning; 4. Other vector systems for E. Coli; 5. Making libraries; 6. Screening libraries; 7. Modification and mutagenesis; 8. Use of cloned DNA; 9. Using other organisms; 10. Examples. Printed Pages: 276 with 131 line diagrams, 9 tables and 131 figures. Size: 190 x 250 Mm. Seller Inventory # 017672
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2. Gene Cloning and Manipulation
Published by
Cambridge University Press, Cambridge, UK
(2007)
ISBN 10: 0521817935
ISBN 13: 9780521817936
New
Hardcover
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Book Description Cambridge University Press, Cambridge, UK, 2007. Hardcover. Condition: New. Dust Jacket Condition: New. Second Edition. Now fully updated to reflect recent advances, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Written for advanced undergraduates, graduates and scientists who want to use this technology, emphasis is placed on the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations. An introduction to the basic biochemical principles is presented first. Then PCR and cloning using E. coli hosts and plasmid, phage and hybrid vectors are described, followed by the generation and screening of libraries and how to modify, inactivate or express cloned sequences. Finally genetic manipulation in a range of other organisms is discussed, including other bacteria, fungi, algae and plants, insects and mammals. A series of 'real-life' biological problems are also presented to enable readers to assess their understanding of the material and to prepare for exams. ?Covers a broad range of host organisms, from E. coli to mammals ?Focuses on the underlying principles thereby equipping the reader to devise appropriate strategies for novel situations and for use in their own research ?Contains example scenarios, which allow the reader to test their understanding of the material and apply it to real-life?research situations Contents Preface; 1. The tools for the job; 2. Polymerase chain reaction; 3. Simple cloning; 4. Other vector systems for E. Coli; 5. Making libraries; 6. Screening libraries; 7. Modification and mutagenesis; 8. Use of cloned DNA; 9. Using other organisms; 10. Examples. Printed Pages: 276 with 131 line diagrams, 9 tables and 131 figures. Size: 190 x 250 Mm. Seller Inventory # 017672
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