Gene Cloning and Manipulation - Hardcover

Howe, Christopher

Hardcover

ISBN 10: 0521817935 ISBN 13: 9780521817936

Publisher: Cambridge University Press, 2007

Synopsis

Updated to reflect advances in the field, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Written for advanced undergraduates, graduates and scientists who want to use this technology, emphasis is placed on the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations. An introduction to the basic biochemical principles is presented first. Then PCR and cloning using E. coli hosts and plasmid, phage and hybrid vectors are described, followed by the generation and screening of libraries and how to modify, inactivate or express cloned sequences. Finally genetic manipulation in a range of other organisms is discussed, including other bacteria, fungi, algae and plants, insects and mammals. A series of 'real-life' biological problems are also presented to enable readers to assess their understanding of the material and to prepare for exams.

About this title

Review

..".will give any new student a foothold in molecular biology....provides a cheap and up-to-date introduction to many fundamental techniques." Jody D. Berry, CSM Newsletter

Book Description

Updated to reflect advances in the field, this introduction provides a broad but concise coverage of recombinant DNA techniques. It is written specifically for students and researchers who want to use this technology, with emphasis placed on understanding the underlying concepts, thereby enabling the reader to devise their own strategies.

Edition details

• Publisher: Cambridge University Press
• Publication date: 2007
• ISBN 10: 0521817935
• ISBN 13: 9780521817936
• Binding: Hardcover
• Language: English
• Edition number: 2
• Number of pages: 276