A Practical Guide to Molecular Cloning - Softcover

Perbal, Bernard

Softcover

ISBN 10: 0471850713 ISBN 13: 9780471850717

Publisher: Wiley–Blackwell, 1988

Synopsis

Presents techniques tested at the Curie Institute and other leading labs and lists all commercially available enzymes, vectors, linkers, and other basic products for ready reference. Offers detailed explanation of protocols, allowing the isolation, cloning, and expression of genes from living species. Presents up–to–date techniques on sequencing, in vitro expression of cloned gene, and use of computers for study of nucleic acids, and is the only book that shows how to isolate DNA–protein complexes and new methods for mutagenesis of cloned genes. Contains 235 figures and 80 tables.

About this title

Synopsis

This new edition of the standard laboratory manual is intended to make molecular cloning faster and easier. It presents techniques tested at the Curie Institute and at other leading laboratories, and lists all commercially available enzymes, vectors, linkers, and other basic products for ready reference. A detailed explanation of protocols is offered, allowing the isolation, cloning, and expression of genes from living species. As well as presenting up-to-date techniques on sequencing, in vitro expression of cloned gene and use of computers for study of nucleic acids, it shows how to isolate DNA-protein complexes and new methods for mutagenesis of cloned genes.

Edition details

• Publisher: Wiley–Blackwell
• Publication date: 1988
• Language: English
• ISBN 10: 0471850713
• ISBN 13: 9780471850717
• Binding: Paperback
• Edition number: 2
• Number of pages: 812