Proteomic Biology Using LC/MS: Large Scale Analysis of Cellular Dynamics and Function (Wiley Series on Mass Spectrometry) - Hardcover

Takahashi, Nobuhiro; Isobe, Toshiaki; Desiderio, Dominic M.; Nibbering, Nico M.

Hardcover

ISBN 10: 0471662585 ISBN 13: 9780471662587

Publisher: Wiley-Interscience, 2007

Synopsis

This is one of the first books to focus on the dynamic aspect of proteomes. The book introduces proteomics to the newcomer, reviews the theoretical aspects of proteomics and its state-of-the art technologies, along with a number of biological applications using "classical" proteomic technology. The book also presents a new concept, the Dynamome, or the expression of a comprehensive molecular set that participates in the whole dynamic process of a series of cellular events.

About this title

About the Author

Nobuhiro Takahashi, PhD, is a Professor of Applied Biological Science in the Department of Agriculture at Tokyo University of Agriculture and Technology.

Toshiaki Isobe, PhD, is a Professor of Biochemistry in the Department of Chemistry at Tokyo Metropolitan University.

From the Back Cover

A comprehensive guide to LC-MS applications that probe the dynamic aspectS of proteomes

The systematic identification and characterization of proteins and proteomes is one of the major interdisciplinary research areas of the life sciences in the post-genomic era. One of the primary goals of current proteomic research is the description of the composition, dynamics, and connections of the multi-protein "modules" and "machineries" that perform a wide range of biological functions in the cell. Proteomic Biology Using LC-MS: Large Scale Analysis of Cellular Dynamics and Function examines the latest LC-MS technologies in depth and explores their application to the analysis of the dynamic aspects of cellular function. After an overview of proteomics and proteomic technologies, it covers:

• The basic aspects of LC-MS technology, including the principle of the method and assembly of the LC-MS system

• Integrated LC-based MS methodologies coupled with quantitative methodologies and/or bioinformatics

• The most recent applications, such as the analyses of signal transduction complexes following stimulation of cells and the analysis of the dynamic processes of ribosome biogenesis

• A new concept, the "Dynamome," or the expression of a comprehensive molecular set that participates in the whole dynamic process of a series of cellular events

Detailed experimental examples help readers explore various applications in action. With comprehensive coverage of LC-MS technologies applicable to the analysis of dynamic cellular functions in proteomic scale, this is a unique reference for investigators and laboratory staffs in academic institutions and industries. It is also a valuable resource for students as well as for scientists in other fields who want to bridge the gap between their specialties and proteomic biology.

From the Inside Flap

A comprehensive guide to LC-MS applications that probe the dynamic aspectS of proteomes

The systematic identification and characterization of proteins and proteomes is one of the major interdisciplinary research areas of the life sciences in the post-genomic era. One of the primary goals of current proteomic research is the description of the composition, dynamics, and connections of the multi-protein "modules" and "machineries" that perform a wide range of biological functions in the cell. Proteomic Biology Using LC-MS: Large Scale Analysis of Cellular Dynamics and Function examines the latest LC-MS technologies in depth and explores their application to the analysis of the dynamic aspects of cellular function. After an overview of proteomics and proteomic technologies, it covers:

• The basic aspects of LC-MS technology, including the principle of the method and assembly of the LC-MS system

• Integrated LC-based MS methodologies coupled with quantitative methodologies and/or bioinformatics

• The most recent applications, such as the analyses of signal transduction complexes following stimulation of cells and the analysis of the dynamic processes of ribosome biogenesis

• A new concept, the "Dynamome," or the expression of a comprehensive molecular set that participates in the whole dynamic process of a series of cellular events

Detailed experimental examples help readers explore various applications in action. With comprehensive coverage of LC-MS technologies applicable to the analysis of dynamic cellular functions in proteomic scale, this is a unique reference for investigators and laboratory staffs in academic institutions and industries. It is also a valuable resource for students as well as for scientists in other fields who want to bridge the gap between their specialties and proteomic biology.

Edition details

• Publisher: Wiley-Interscience
• Publication date: 2007
• Language: English
• ISBN 10: 0471662585
• ISBN 13: 9780471662587
• Binding: Hardcover
• Edition number: 1
• Number of pages: 272