Offering an introductory course on the language of gene cloning, this text covers microbial, plant and animal systems. The essential concepts in biology relevant to the understanding of gene cloning are presented in an accessible manner. The book begins by outlining the basic principles of biology and aspects of genetics relevant to the concepts of gene cloning. The next section explores the common techniques and approaches used in gene cloning. The author then discusses various applications in agriculture and food, and in medicine and related areas. The practical uses of gene cloning are illustrated, with specific examples from the literature to demonstrate how gene cloning is conducted utilizing the concepts developed in previous sections. Review questions at the end of each chapter provide self-testing, allowing the reader to assess their understanding of the materials. This book should be of interest to researchers, academics, graduate students in food science, agriculture, nutrition, biology, microbiology, genetics, zoology, cell biology, plant biology, neuroscience, aquaculture and fisheries, and entomology.
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The ABCs of Gene Cloning, Second Edition
Dominic W. S. Wong
The ABCs of Gene Cloning serves as a clear and easy-to-use text that offers the reader an introductory course on the language of gene cloning. It assumes no prior knowledge on the part of the reader, while emphasizing the 'nuts and bolts' of gene cloning. The ABCs of Gene Cloning enables the reader to distinguish between a gene and a DNA, to read and write a gene sequence, to talk intelligently about gene cloning, to attend seminars without getting completely lost, and to comprehend materials in scientific publications.
The ABCs of Gene Cloning contains updated information throughout, a tutorial on how to read a gene sequence, new topics on animal cloning and human genome sequencing, and rewritten chapters on human gene therapy, gene targeting, and DNA typing. Part I of this book outlines the very basic principles of biology and genetics, with emphasis only on the aspects relevant to gene cloning. The intent is to provide sufficient background materials without burdening the reader with unwarranted details. Part II describes the common techniques and approaches of gene cloning used in microbial, plant and mammalian systems. It includes topics ranging from the basic mechanics of DNA manipulation, vector construction, to genomic analysis, which is still a very new and rapidly evolving field. Part III presents selected applications of major impact on biomedicine, agriculture and related areas. Case examples are used to demonstrate how gene cloning is conducted and applied in biomedicine and agriculture.
Key features:
The ABCs of Gene Cloning is essential reading for students in biological science, biomedicine, and related disciplines, as well as others who have an interest in gaining proficiency in the language of gene cloning. The book assumes no prior knowledge of the subject, and uniquely provides the nuts and bolts for learning the art of gene cloning in a simple, logical, and thorough manner.
The Author:
Dominic W. S. Wong, Ph.D. is a scientist at the Western Regional Research Center, Agricultural Research Service, U. S. Department of Agriculture. Dr. Wong is also the author of Mechanisms and Theory in Food Chemistry and Food Enzymes: Structure and Mechanism, and co-editor of Handbook of Food Enzymology.
Dominic W. S. Wong, Ph.D., is a Research Chemist at the Western Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture.
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