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Excerpt from A Biochemical Study of the Toxin of Bacillus Pyocyaneus: Thesis
The physiological properties of the toxin of Bacillus pyocyaneus have been most often studied on the tissues of mammalian, warm-blooded animals. In this study the action of this toxin upon an animal of the cold-blooded type-paramoecium-was investigated. The object was to ascertain the general protoplasmic reaction to the toxin rather than that of any Specialized group of cells. This purpose was still further favored by the selection of a highly generalized form of protoplasm, that of a free-living unicellular organism.
Furthermore a successful effort was made to produce a toxin of constant i. E. Of easily reproducible properties. A satisfactory method was sought for raising the bacterial cultures and especially of separating the characteristic toxin from.other toxic chemical products of the bacillus. The comparability of different stocks of toxin thus prepared by the same method was further increased by the measurement of the strength of the toxin against the same practically uniform standard consisting of paramoecia raised by a con stant method.
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