Principles of Genomics and Proteomics: A Technical Guide - Softcover

Ahmad Mir; Shafi; Zargar

Softcover

ISBN 10: 0323990452 ISBN 13: 9780323990455

Publisher: Elsevier, 2023

Synopsis

Principles of Genomics and Proteomics is the perfect reference for graduate students and researchers in these areas to understand its principles and execute precise and reproducible experiments. Following an introductory chapter, the book dives into proper research, including genome mapping. Experiments covered in the book span from Sangers Sequencing, Shotgun sequencing, SAGE analysis, DNA footprinting, Gel retardation, ChIP, and protein resolution methods, including PAGE, 2D gel electrophoresis and isoelectric focusing. Biophysical techniques are also described in detail, including ultraviolet and visible light spectroscopy, fluorescence spectroscopy, NMR and X-ray diffraction.

A final proteome analysis is dedicated to functional analysis. Other chapters cover applications of omics technologies broadly. This book is the perfect reference for genetics labs around the world. Graduate students will benefit from the structured and detailed coverage of methods and established researchers will benefit from the book for staff training in research and may find it particularly helpful in enhancing reproducibility of experiments.

• Provides a comprehensive accounting of the technical aspects of genomic and proteomic analysis and applications in living organisms
• Includes detailed and standardized coverage of basic research methods in areas covered
• Presents advanced applications of genomics and proteomics that are highlighted with major significance in medical and agricultural fields

About this title

About the Authors

Rakeeb Ahmad Mir is Assistant Professor, Department of Biotechnology, School of Life Sciences, Central University of Kashmir, Science Campus, Nunner, Ganderrbal, Srinagar, Jammu & Kashmir, India. He is having 6 years of Research and Teaching Experience in different subjects of Biotechnology. His research interests include Molecular taxonomy, Nematode genomics and basic research in proteomics. In addition, he is deeply involved in teaching courses such as, Cell Biology, Signal transduction and Cancer Biology, Molecular Biology, Genomics and Proteomics and Nematode genomics at University level. Dr Rakeeb has published various research and review papers, book chapters on Molecular taxonomy of nematodes, Proteomic techniques and stress tolerant crop plants. Since he has considerable experience in the related fields pertaining to the title of this book.

Dr. Sheikh Mansoor Shafi currently serves as a postdoctoral researcher at the Phenomics Laboratory within Jeju National University in South Korea. His primary research focuses on high-throughput phenomics, remote sensing and precision agriculture. Prior to this role, he held positions as a Postdoc Research Associate at SKIMS Srinagar and as a Project Associate-I at CSIR-Indian Institute of Integrative Medicine Jammu (IIIM). Throughout his academic journey, Dr. Mansoor has had the opportunity to work in reputed laboratories, including the Transcriptomics Labs at SKUAST Kashmir and the Department of Biotechnology and Pathology at Central Institute of Temperate Horticulture (CITH) Rangreth Srinagar. His educational background comprises a BSc degree from SP College of Science (University of Kashmir), an MSc degree from HNBGU Uttarakhand, and a Ph.D. degree from Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu.

Sajad Majeed Zargar, Ph.D, is currently an Assistant Professor at Sher-e-Kashmir University of Sciences & Technology in India and visiting Professor at University of Padova, Italy. Dr. Zargar is the recipient of the CREST overseas fellowship from DBT, the Goho grant from Govt. of Japan, and the Erasmus Fellowship from European Union. He is also a member and representative of INPPO (International Plant Proteomics Organisation). His editorial activities and scientific memberships include publishing research and review articles in international journals and is also reviewer.

From the Back Cover

Principles of Genomics and Proteomics is the perfect reference for graduate students and researchers in these areas to understand its principles and execute precise and reproducible experiments.

Following an introductory chapter, the book dives into proper research, including genome mapping. The following sections cover in detail, following a detailed template, genomic and proteomic assays. Experiments present in the book span from Sangers Sequencing, Shotgun sequencing, SAGE analysis, DNA footprinting, Gel retardation, ChIP, moving then to protein resolution methods including PAGE, 2D gel electrophoresis and isoelectric focusing. Biophysical techniques are described in detail, including ultraviolet and visible light spectroscopy, fluorescence spectroscopy, NMR and X-ray diffraction. A final proteome analysis is dedicated to functional analysis. The final chapter covers applications of omics technologies broadly.

Principles of Genomics and Proteomics is the perfect reference for genetics labs around the world. Graduate students will benefit from the structured and detailed coverage of methods. Established researchers can benefit from the book for staff training in research and may find it particularly helpful in enhancing reproducibility of experiments.

Edition details

• Publisher: Elsevier
• Publication date: 2023
• Language: English
• ISBN 10: 0323990452
• ISBN 13: 9780323990455
• Binding: Paperback
• Edition number: 1
• Number of pages: 274