Principles of Fluorescence Spectroscopy - Hardcover

Hardcover

ISBN 10: 0306412853 ISBN 13: 9780306412851

Publisher: Kluwer Academic / Plenum Publishers, 2003

Synopsis

Fluorescence methods are being used increasingly in biochemical, medical, and chemical research. This is because of the inherent sensitivity of this technique. and the favorable time scale of the phenomenon of fluorescence. 8 Fluorescence emission occurs about 10- sec (10 nsec) after light absorp­ tion. During this period of time a wide range of molecular processes can occur, and these can effect the spectral characteristics of the fluorescent compound. This combination of sensitivity and a favorable time scale allows fluorescence methods to be generally useful for studies of proteins and membranes and their interactions with other macromolecules. This book describes the fundamental aspects of fluorescence. and the biochemical applications of this methodology. Each chapter starts with the -theoreticalbasis of each phenomenon of fluorescence, followed by examples which illustrate the use of the phenomenon in the study of biochemical problems. The book contains numerous figures. It is felt that such graphical presentations contribute to pleasurable reading and increased understand­ ing. Separate chapters are devoted to fluorescence polarization, lifetimes, quenching, energy transfer, solvent effects, and excited state reactions. To enhance the usefulness of this work as a textbook, problems are included which illustrate the concepts described in each chapter. Furthermore, a separate chapter is devoted to the instrumentation used in fluorescence spectroscopy. This chapter will be especially valuable for those perform­ ing or contemplating fluorescence measurements. Such measurements are easily compromised by failure to consider a number of simple principles.

About this title

Review

`... recommended without reservation both to the novice and to the expert in fluorescence.'
Analytical Biochemistry
`In addition to its use as a student text, it should be a particularly valuable reference for those involved in biochemical research.'
Chemistry in Britain

From the Back Cover

Principles of Fluorescence Spectroscopy, 3rd edition

, 3rd edition

Joseph R. Lakowicz

The third edition of the established classic text reference, Principles of Fluorescence Spectroscopy, will enhance upon the earlier editions' successes. Organized as a textbook for the learning student or the researcher needing to acquire the core competencies, Principles of Fluorescence Spectroscopy, 3e will maintain the emphasis on basics, while updating the examples to include recent results from the literature. The third edition also includes new chapters on single molecule detection, fluorescence correlation spectroscopy, novel probes and radiative decay engineering.

This full-color textbook features the following:

• Problem sets following every chapter
• Glossaries of commonly used acronyms and mathematical symbols
• Appendices containing a list of recommended books which expand on various specialized topics
• Sections describing advanced topics will indicate as such, to allow these sections to be skipped in an introductory course, allowing the text to be used for classes of different levels
• Includes CD-ROM of all figures in a low-res format, perfect for use in instruction and presentations

Principles of Fluorescence Spectroscopy, 3rd edition, is an essential volume for students, researchers, and industry professionals in biophysics, biochemistry, biotechnology, bioengineering, biology and medicine.

About the Author:

Dr. Joseph R. Lakowicz is Professor of Biochemistry at the University of Maryland School of Medicine, Baltimore, and Director of the Center for Fluorescence Spectroscopy. Dr. Lakowicz has published over 400 scientific articles, has edited numerous books, holds 16 issued patents, and is the author of the widely used text, Principles of Fluorescence Spectroscopy now in its 3rd edition.

 

Edition details

• Publisher: Kluwer Academic / Plenum Publishers
• Publication date: 2003
• Language: English
• ISBN 10: 0306412853
• ISBN 13: 9780306412851
• Binding: Hardcover
• Number of pages: 510
• Editor: Lakowicz Joseph R.