Synopsis
This historic book may have numerous typos and missing text. Purchasers can usually download a free scanned copy of the original book (without typos) from the publisher. Not indexed. Not illustrated. 1913 edition. Excerpt: ...layer of toluol is placed also on the outside. Controls should be prepared in the same way, consisting of the patient's serum alone, and of the placental tissue alone respectively. Place in the incubator for 12-16 hours. Now remove 10 c.c. of the fluid outside of the shell, by means of a pipette thrust below the level of the toluol, and test this for peptone with one or both of the reagents described above. When triketohydrinden hydrate is to be used, it is especially important that as little toluol as possible be transferred to the test tube, as overheating is then apt to occur. A glass rod may be placed in the test tube to prevent bumping. A positive result is indicated when the dialy-sate gives a positive reaction for peptone. The method has been verified by K. Franz 23 and by Frank and Heimann.24 The polariscope method of Abderhalden, which is given in detail in the monograph first cited above, is here omitted because it is so complicated as to fall outside the domain of the usual clinical laboratory. Moro's Alexin Test25 (Estimation of the Complement Content of a Patient's Serum.)--Principle.--The patient's serum, in increasing quantities, is mixed with a fixed quantity of washed erythro-cytes, and an amount of inactivated (i.e., complement free), immune serum (amboceptor serum), sufficient to cause solution of the red cells. After incubation for 2 hours, the degree of haemolysis is noted. The amount of the patient's serum required to bring about haemolysis affords an approximate measure of the complement (alexin) content of the serum. Apparatus.--(1) A 10 per cent. suspension in physiological salt solution of washed sheep's blood-cells. (2) Antisheep immune serum, of high potency, and inactivated, to serve as amboceptor. "Franz,...
"synopsis" may belong to another edition of this title.
