This convenient laboratory manual covers the theory and application of all the commonly used FISH procedures for both the research and clinical service laboratory. Readers are provided with the theoretical principles which underlie each procedure, and are then guided through the protocols in a systematic, easy to follow manner. Because FISH procedures are particularly sensitive to background and subtle variations in sample quality, the trouble-shooting sections are invaluable to the beginner interested in developing FISH capabilities in their laboratory. Areas covered include probe production and labeling; FISH gene mapping; murine, fiber, and RNA FISH; CGH and microchips; 3D FISH; SKY and M-FISH; FISH and microarrays; and FISH in clinical cytogenetics.
"synopsis" may belong to another edition of this title.
As a resource for both the researcher and the clinician, this book should serve admirably. It should be a welcome addition to any technical library. (Proteomics)
... offers an excellent array of protocols aimed at the laboratory scientist. (Proteomics)
... the book does an excellent job of describing purification and enzymatic labeling techniques of probe DNA, and offers a comprehensive list of sources for various FISH probes and reagents. (Proteomics)
... useful as a laboratory manual. (Proteomics)
Barbara G Beatty is at Health Sciences Research Facility, University of Vermont, USA. Sabine Mai is at Manitoba Institute of Cell Biology, Winnipeg, Canada.
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