With the 'post genomics' era comes an increasing demand for the techniques of cell biology, critical to interpreting the function and location of the cell's myriad proteins and macromolecules. In response, this second edition of Plant Cell Biology balances established techniques, including classical histochemistry and electron microscopy, with new developments in the field. The book covers a substantial range of methods for working on living cells, including the application of fluorescent probes, cytometry, expression systems, the use of green fluorescent protein, micromanipulation and electrophysiological techniques. Also featured are chapters on macromolecular location procedures involving immunocytochemistry and in situ hybridisation, and the book concludes with a range of biochemical techniques for the isolation of cytoplasmic organelles. The book provides advanced students, postgraduates and researchers in the plant sciences with an invaluably comprehensive guide to the ever-growing field of plant cell biology.
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... an excellent guide for advanced students, postgraduates and researchers to handle techniques in plant cell biology. (Journal of Plant Physiology)
The layout of the book is clear, with highly informative illustrations ... For anyone curious about cell biology, this book provides an excellent introduction to the major relevant techniques and works well as a laboratory manual. (Trends in Plant Science, 2002)
Chris Hawes is at Oxford Brookes University. Beatrice Satiat-Jeunemaitre is at Institut des Sciences Vegetales, Gif-sur-Yvette.
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