Nuclear Receptors: A Practical Approach: 207 (Practical Approach Series) - Softcover

Softcover

ISBN 10: 0199637423 ISBN 13: 9780199637423

Publisher: Oxford University Press, U.S.A., 1995

Synopsis

The steroid or nuclear receptor superfamily is an important group of transcription factors that is studied by a large and varied number of basic and clinical researchers. Nuclear Receptors: A Practical Approach provides these researchers an invaluable guide to do so. With an international list of contributors and examples of over 70 tried and true protocols, this book offers substantial insight into the growing family of biologically important receptors. Its chapters address a variety of factors, including the evolutionary biology of the super family and how to clone and characterize new receptors. Also addressed are topics such as identification of the ligands of novel receptors, the kinetic analysis of receptor interactions, and the functional characterization of coactivators using microinjection. Various sections throughout the book cover receptor phosphorylation, ligand regulated transcription, and hormone resistance syndromes. The book concludes with a description of yeast as a model system for looking at receptor function, and the final chapter shows how heterologous proteins can be regulated by fusion to the hormone binding domain of a receptor. Nuclear Receptors: A Practical Approach is a useful tool for any researcher studying members of the super family, and it is a must for all biomedical researchers.

About this title

Review

"... An authoritative and wide-ranging 'methods' book in a distinguished series"Aslib Book Guide

"Dr Picard has done an excellent job in bringing together a range of exciting techniques that are currently being used to futher our understanding of this fascinating and ever growing family of 'Nuclear Recptors"Cell Biology International

Synopsis

The steroid / nuclear receptor superfamily is a large and growing group of transcription factors that are studied by a large and varied number of basic and clinical researchers. The first two chapters describe the evolutionary biology of the superfamily and explain how to clone and characterize new receptors. Chapter 3 shows how to identify the ligands of novel receptors and chapter 4 explains the kinetic analysis of receptor interactions. In chapter 5, the reader is guided through the functional characterization of coactivators using microinjection. The next section covers receptor phosphorylation, ligand regulated transcription, and hormone resistance syndromes. Chapter 9 describes the in vitro assembly of Hsp90 complexes and chapter 10 explains yeast as a model system for looking at receptor function. The final chapter shows how heterologous proteins can be regulated by fusion to the hormone binding domain of a receptor. Nuclear Receptors: A Practical Approach is a comprehensive guide to studying members of the superfamily and will be invaluable to all researchers old and new.

Edition details

• Publisher: Oxford University Press, U.S.A.
• Publication date: 1995
• Language: English
• ISBN 10: 0199637423
• ISBN 13: 9780199637423
• Binding: Paperback
• Edition number: 2
• Number of pages: 306
• Editor: Picard Didier