Protein Purification Applications: A Practical Approach (Practical Approach Series)

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9780199636723: Protein Purification Applications: A Practical Approach (Practical Approach Series)

Proteins are an integral part of molecular and cellular structure and function and are probably the most purified type of biological molecule. In order to elucidate the structure and function of any protein it is first necessary to purify it. Protein purification techniques have evolved over the past ten years with improvements in equipment control, automation, and separation materials, and the introduction of new techniques such as affinity membranes and expanded beds. These developments have reduced the workload involved in protein purification, but there is still a need to consider how unit operations linked together to form a purification strategy, which can be scaled up if necessary. The two Practical Approach books on protein purification have therefore been thoroughly updated and rewritten where necessary. The core of both books is the provision of detailed practical guidelines aimed particularly at laboratory scale purification. Information on scale-up considerations is given where appropriate. The books are not comprehensive but do cover the major laboratory techniques and common sources of protein. Protein Purification Techniques focuses on unit operations and analytical techniques. It starts with an overview of purification strategy and then covers initial extraction and clarification techniques. The rest of the book concentrates on different purification methods with the emphasis being on chromatography. The final chapter considers general scale-up considerations. Protein Purification Applications describes purification strategies from common sources: mammalian cell culture, microbial cell culture, milk, animal tissue, and plant tissue. It also includes chapters on purification of inclusion bodies, fusion proteins, and purification for crystallography. A purification strategy that can produce a highly pure single protein from a crude mixture of proteins, carbohydrates, lipids, and cell debris to is a work of art to be admired. These books (available individually or as a set) are designed to give the laboratory worker the information needed to undertake the challenge of designing such a strategy.

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About the Author:

Simon Roe is at AEA Technology Products and Systems, Biosciences, Harwell.

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"Describes strategies and detailed practical procedures for laboratory scale protein purification from common sources: mammalian cell culture, microbial cell culture, animal tissue, plant tissue, and milk. Chapters are included on the purification of inclusion bodies, fusion proteins, and proteins for crystallography. Intended for biotechnology researchers. Includes a list of suppliers."--SciTech Book News


"This book is written for the lab worker. . . What I found most appealing in the book was the inclusion of about 46 detailed protocols describing actual procedures for protein purification. A bonus for the reader is a "List of suppliers" with contact information for about 50 companies that are primarily in the United Kingdom and the USA."--Biochemsitry and Molecular Biology Education


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