High Resolution Chromatography: A Practical Approach: 204 (Practical Approach Series) - Hardcover

Hardcover

ISBN 10: 0199636494 ISBN 13: 9780199636495

Publisher: OUP Oxford, 1999

Synopsis

The molecular biology revolution has required the development of new chromatographic techniques and the optimization of original techniques to give reasonable quantities of protein at high resolutions. The aim of this volume is to provide the necessary information in most experimental situations to enable rapid and effective purification. The first four chapters deal with the instrumental aspects of high resolution chromatography starting with the initial clean up steps prior to separation in chapter 1. Chapter 2 deals with microscale techniques, then chapter 3 describes the detector technologies that can determine information about the separated molecules. The final chapter in this section cover capillary electrophoresis and its associated techniques. The remaining chapters cover a range of chromatographic procedures based on the interaction of a specific ligand with its target protein or other macromolecule. Some chapters cover non-specific interactions using peptides, inhibitors, and antibodies as the affinity ligand while others focus on specific groups of molecules : oligosaccharides and glycosylated proteins, nucleotide-binding proteins, proteins binding free and chelated metal ions, and DNA binding proteins.

About this title

Product Description

High Resolution Chromatography The molecular biology revolution has created a need for new chromatographic techniques and the optimization of older ones to give reasonable quantities of protein at high resolutions. This text aims to provide the information for most experimental situations to enable rapid, effective purification. Full description

Review

"Laboratory workers will find this a useful guide to the development of effective purification protocols"Aslib Book Guide"

Edition details

• Publisher: OUP Oxford
• Publication date: 1999
• Language: English
• ISBN 10: 0199636494
• ISBN 13: 9780199636495
• Binding: Hardcover
• Number of pages: 333
• Editor: Millner Paul