High Resolution Chromatography: A Practical Approach: 204 (Practical Approach Series) - Softcover

Softcover

ISBN 10: 0199636486 ISBN 13: 9780199636488

Publisher: Oxford University Press, USA, 1999

Synopsis

Isolating and purifying proteins is a key step for a wide range biological experiments, and researchers require quick and reliable techniques. This practical volume fits this need. The first section, devoted to techniques and equipment, covers initial preparation, microscale techniques, detector technologies, and capillary electrophoresis and its associated techniques. The second section then covers a range of chromatographic procedures based on the interaction of a specific ligand with its target protein or other macromolecule. Some chapters cover non-specific interactions using peptides, inhibitors, and antibodies as the affinity ligand, while others focus on specific groups of molecules, including oligosaccharides and glycosylated proteins, nucleotide-binding proteins, proteins binding free and chelated metal ions, and DNA-binding proteins.

About this title

Review

"Laboratory workers will find this a useful guide to the development of effective purification protocols"Aslib Book Guide"

Synopsis

The molecular biology revolution has required the development of new chromatographic techniques and the optimization of original techniques to give reasonable quantities of protein at high resolutions. The aim of this volume is to provide the necessary information in most experimental situations to enable rapid and effective purification. The first four chapters deal with the instrumental aspects of high resolution chromatography starting with the initial clean up steps prior to separation in chapter 1. Chapter 2 deals with microscale techniques, then chapter 3 describes the detector technologies that can determine information about the separated molecules. The final chapter in this section cover capillary electrophoresis and its associated techniques. The remaining chapters cover a range of chromatographic procedures based on the interaction of a specific ligand with its target protein or other macromolecule.

Some chapters cover non-specific interactions using peptides, inhibitors, and antibodies as the affinity ligand while others focus on specific groups of molecules : oligosaccharides and glycosylated proteins, nucleotide-binding proteins, proteins binding free and chelated metal ions, and DNA binding proteins.

Edition details

• Publisher: Oxford University Press, USA
• Publication date: 1999
• Language: English
• ISBN 10: 0199636486
• ISBN 13: 9780199636488
• Binding: Paperback
• Number of pages: 334
• Editor: Millner Paul