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Synopsis
Part of a mini-series on the synthesis and processing of proteins, this volume details the expression of cloned D.N.A. and R.N.A. templates in all of the major in vivo and in vitro systems. The in vivo systems covered are cultured mammalian cells, the yeasts Sacchoromyces cerevisiae and Pichia pastoris, baculovirus, Xenopus oocytes, and prokaryotic cells. It also covers cell-free systems of both eukaryotes and prokaryotes, including prokaryotic systems that offer coupled transcription-translation. The volume concludes with a chapter on monitoring protein expression.
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Review
' The earlier volume on Transcription and Translation has been well used in my laboratory, and I am sure this update will become equally well thumbed' Microbiology Today
Synopsis
Protein Expression: A Practical Approach and its companion volume Post-translational Modification: A Practical Approach complete the mini-series of Practical Approach books covering the synthesis and subsequent processing of proteins. Protein Expression: A Practical Approach details the expression of cloned DNA or RNA templates in all the major in vivo and in vitro systems. The in vivo systems covered are cultured mammalian cells, the yeasts Saccharomyces cerevisiae and Pichia pastoris, baculovirus, Xenopus oocytes, and prokaryotic cells. Cell-free systems of both eukarytotes and prokaryotes are described, including the prokaryotic systems that offer copuled transcription- translation. There is also a chapter on monitoring protein expression. The post- translational fate of proteins is covered in Post-Translational Processing: A Practical Approach.
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- PublisherOxford University Press, U.S.A.
- Publication date1999
- ISBN 10 0199636230
- ISBN 13 9780199636235
- BindingPaperback
- LanguageEnglish
- Number of pages304
- EditorHiggins S. J.
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Protein Expression: A Practical Approach (Practical Approach Series)
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Protein Expression: A Practical Approach (Practical Approach Series)
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Protein Expression
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