HIV: A Practical Approach Volume 2: Biochemistry, Molecular Biology, and Drug Discovery (The Practical Approach Series): 157 - Softcover

Softcover

ISBN 10: 019963498X ISBN 13: 9780199634989

Publisher: Oxford University Press, USA, 1995

Synopsis

HIV Volume 2 explores the biochemistry and molecular biology of HIV. Methods for cloning and expressing most of the viral proteins are described as well as assays for studying enzyme function in vitro. It also includes a section on recent approaches to drug discovery.

Contents: The HIV life cycle; Reverse transcriptase; Ribonulease H; Integrase; Protease; Expression of gag, envelope and other HIV antigens using Bacculovirus; Envelope expression and purification; HIV envelope glyocoprotein/CD4 interactions - studies using recombinant vaccinia virus vectors; RNA binding assays for the regulatory proteins tat and rev; Cellular and cell-free assays for tat; Cellular assays for rev; DNA binding proteins interacting with the viral LTR; Nef; Vpu; Vif; Cellular assays for antiviral drugs; Inhibition of HIV by ribozymes and antisense oligonucleotides; Gene therapy approaches to the inhibition of HIV

About this title

Review

HIV: a Practical Approach is the latest two-volume offering from the Practical Approach series and provides the first comprehensive methodology for the HIV field. As such, it can be recommended strongly to all active bench scientists in the field as well as to desk-bound research directors who wish to keep in touch ... well worth the effort. (James C. Neil, University of Glasgow Veterinary School, Molecular Medicine Today, October 1996)

The two volumes are of exceptional value for laboratory workers studying the many different aspects of HIV and HIV disease. At an affordable price, these two volumes provide a wealth of information on standard techniques and on more sophisticated approaches for the study of the interaction between cellular and viral proteins. It is a comprehensive manual which serves as a solid basis for all investigators who are exploring this complex virus and the complex pathogenesis of HIV disease. Prferably in the form of multiple copies, it deserves a place on the bookshelf of any laboratory working with HIV. (M.A. Koch, Zentralblatt fur Bakteriologie 1996)

Synopsis

AIDS is one of the most pressing medical emergencies of our era. Despite arguments to the contrary, the majority of AIDS researchers have agreed that there is a causal link between HIV infections and AIDS. However, it is now 12 years since the discovery of HIV and we are still a long way from understanding and controlling AIDS. HIV 1 and HIV 2 are guides to studying HIV in the laboratory. HIV 1 describes studies of the virus and infected cells and covers techniques for handling HIV safely, for studying viral entry, replication, and neutralization, and basic immunological techniques. HIV 2 explores the biochemistry and molecular biology of HIV. Methods for cloning and expressing most of the viral proteins are described, plus assays for studying enzyme function in vitro. It also includes a section on recent approaches to drug discovery. HIV research is highly multidisciplinary so a complete compendium of all related methods would have been unreadable. Instead, theses two volumes focus on the specialized techniques which are unique to studies of HIV.

The books have been designed so that a novice entering HIV research can find clear instructions about how to perform standard experiments, such as assays for reverse transcriptase or antibodies to HIV. In addition the books include many novel methods which are described in detail for the first time.

Edition details

• Publisher: Oxford University Press, USA
• Publication date: 1995
• Language: English
• ISBN 10: 019963498X
• ISBN 13: 9780199634989
• Binding: Paperback
• Number of pages: 356
• Editor: Karn Jonathan