Neural Cell Culture: A Practical Approach (Practical Approach Series): 165 - Softcover

Softcover

ISBN 10: 019963484X ISBN 13: 9780199634842

Publisher: Oxford University Press, USA, 1996

Synopsis

The immense complexity and cellular heterogeneity of the vertebrate nervous system makes it difficult to study many types of neural processes at a cellular and molecular level. In recent years, the establishment of cell culture model systems for almost every region and cell type in the nervous system has been crucial in facilitating such studies and in heralding some key breakthroughs in our understanding of the underlying mechanisms.

Neural Cell Culture brings together methods for preparing cultures from a diverse range of neural tissues and cells, from both the central and peripheral nervous systems of mammalian and avian species. Important features of this volume are the clearly illustrated dissection guides accompanying each chapter, methods for enriching and purifying distinct cell types, and guidance on optimizing cell viability and growth. Each chapter includes valuable step-by-step protocols, hints and tips for success, advice, and background information.

About this title

Review

Sixteen chapters provide detailed methodological protocols on how to culture different types of neural tissue ... These chapters have been written by experts who have extensive experience with the methods in their own laboratories; hence, they typically reflect the product of years of experience with variants of the protocols described. The book is very accessible and easy to use. It is well organized, and indexed ... Nothing teaches like experience, and at least this book will give you some ideas about the experience of others that will help to guide your progress. For the neuroscientist engaged in neural cell culture this manual may be useful as a general reference, and for anyone who has immediate need for a particular chapter, this manual could indeed prove a very helpful resource. (R.E. Strecker, Harvard Medical School,)

This practical book describes tried and tested methods for preparing cultures from a diverse range of tissues and cells from the central and peripheral nervous systems of mammals and birds. (Aslib Book Guide, vol.61, no.9, September 1996.)

About the Author

James Cohen is at Guy's Hospital, London. Graham Wilkin is at Imperial College, London.

Edition details

• Publisher: Oxford University Press, USA
• Publication date: 1996
• Language: English
• ISBN 10: 019963484X
• ISBN 13: 9780199634842
• Binding: Paperback
• Number of pages: 272
• Editor: Cohen James