Plasmids: A Practical Approach (The Practical Approach, No 138) (Practical Approach Series) - Softcover

9780199634446: Plasmids: A Practical Approach (The Practical Approach, No 138) (Practical Approach Series)

Softcover

ISBN 10: 0199634440 ISBN 13: 9780199634446

Publisher: Oxford University Press, USA, 1994

This completely updated and revised second edition of Plasmids: A Practical Approach provides the researcher with detailed protocols for studying bacterial plasmids and for using plasmids and phagemids as vectors. With many new sections and protocols, it is the most up-to-date reference describing the techniques for the study of plasmid replication and maintenance, the fundamental methods for purifying plasmids and introducing them into cells, and the protocols for the mutagenesis and analysis of plasmid-encoded products. The book features further techniques for the study of plasmids and the use of plasmid vectors in such important groups of bacteria as animal and plant pathogens harboring virulence plasmids, Streptomyces, and lactococci. Plus, the book describes the use of plasmid composite vectors for expression of cDNA cloning, and much more. It is a invaluable reference for molecular biologists, microbiologists, and biotechnology researchers. It includes detailed protocols for studying bacterial plasmids and for using both plasmids and phagemids as vectors, techniques for studying fundamental aspects of plasmids, such as replication, are included, as well as methods for using plasmid vectors in important groups of bacteria. Also described are protocols for using phagemids, which are extremely useful tools for genetic engineering.

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Review

'this volume provides practical guidance on plasmid replication and maintenance, methods for purifying plasmids and introducing them into cells ... essential reading for all those using recombinant DNA techniques'Aslib Book Guide, Vol. 59, No. 4, April 1994

The book will be essential reading for all those using recombinant DNA techniques. (Aslib Book Guide, Vol. 59, No. 4, April 1994)

Synopsis

This work is designed to provide research workers with detailed protocols for studying bacterial plasmids, and for using both plasmids and phagemids as vectors. The techniques for studying plasmid replication and maintenance are described in detail, as are fundamental methods for purifying plasmids and for introducing them into cells. Also included are protocols for mutagenesis, and for analyzing plasmid-encoded products. Other chapters focus on techniques for studying plasmids and for using plasmid vectors in particularly important groups of bacteria - animal and plant pathogens harbouring virulence plasmids, streptomyces and lactococci. Plasmid composite vectors and phagemids of various kinds are useful for expression, cDNA cloning and other genetic manipulations. The protocols for using them, as well as important yeast shuttle vectors are described in detail.

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