PCR is now one of the most widely used basic molecular biology techniques and is an indispensable research tool for the molecular biologist. The basic PCR technique provides the cornerstone for in vitro DNA amplification, upon which further features can be built to expand the range of PCR-based applications. This volume sets out to address some of the new applications of PCR, including direct sequencing, mRNA quantitation and expression, genomic DNA mapping and mutation analysis.
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This book gives a good insight into the more advanced PCR technology avaiable and will certainly be a useful addition to laboratories wishing to expand their PCR repertoire. (Dr Amanda Evans, Pharma Research, CIBA AG, Basel, Cell Biology International, 1996, Vol. 20, No. 6)
B. D. Hames, Department of Biochemistry, both at the University of Leeds. G.R. Taylor, Regional DNA Laboratory, Leeds.
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