Gene probes find a wide variety of uses in molecular biology, and are essential tools for genetic research. The advent of solid supports, new D.N.A. labeling techniques, and P.C.R. technology has created a need for an up-to-date practical manual in this area. Gene Probes (Applications), describes techniques for specific research goals. It covers: hybridization strategies; choosing probes; screening and analysis of D.N.A. libraries, including the use of P.C.R.; blotting techniques, gene targeting; and analysis of human genetic mutations. Its companion volume, Gene Probes 1, describes the wide rage of techniques now available for the purification, synthesis, labeling, detection, and quantitation of probes.
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Gene probes find a wide variety of uses in molecular biology, and are essential tools for genetic research. This volume describes some of their many applications, from the study of nucleic acids, through blotting techniques and gene targeting, to the analysis of human genetic mutations. A companion volume covers the preparation of these probes. The volumes aim to contribute to a growing field of research.
S. J. Higgins is at University of Leeds.
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