The preparation of gene probes is a core application in molecular biology, creating essential tools for genetic research. A companion volume, Gene Probes 2, covers applications of the probes. The two books are timely additions to the series, as this is a progressive field which continues to grow in importance.
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This book is intended for use as a laboratory manual, and contains practical assistance to the best of the current methodology within its subject area. (International Journal of Biochemistry and Cell Biology, Vol. 28, No. 7, 1996)
a very practical book (Cellular & Molecular Biology, Vol. 42, No. 3, 1996)
The preparation of gene probes is a core application in molecular biology creating essential tools for genetic research. This volume together with its forthcoming companion volume, Gene Probes 2, provides a timely and important resource for all researchers in this field. Gene Probes 1 focuses on the wide range of techniques now available for the purification, synthesis, labelling, detection, and quantitation of probes, including antibody probes. Gene Probes 2 will concentrate on describing techniques for specific research goals. Contents List: Strategies for using gene probes; Preparation of nucleic acids for use as probes; DNA radiolabelling and detection; Non-radioactive labelling of DNA probes; Preparation of oligodeoxynucleotide probes; Probe preparation by PCR; Synthesis of labelled riboprobes; Antibody probes
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