Crystallization of Nucleic Acids and Proteins: A Practical Approach: 86 (Practical Approach Series) - Softcover

Softcover

ISBN 10: 0199632464 ISBN 13: 9780199632466

Publisher: IRL Press, 1992

Synopsis

In the last decade crystallography has emerged as a key tool for understanding the structures of biological macromolecules, yet the crystallization techniques themselves have been perceived as difficult to perform. This book provides the first detailed and rational guide to producing crystals of proteins and nucleic acids for diffraction studies. Standard crystallization methods are given, along with procedures for the preparation and handling of macromolecules. Detailed laboratory protocols and the underlying theoretical background are brought together, providing researchers with the conceptual and methodological tools necessary to exploit these powerful techniques.

About this title

Review

'an invaluable contribution to the literature in this research area ... it will be most useful for students and beginners in the field of crystallization ... this very up-to-date, comprehensive text ... is written and presented with great clarity. I can strongly recommend it as a standard text on crystallization that no laboratory involved in the field should be without.'Lesley F. Lloyd, Imperial College of Science, Technology and Medicine, Tibtech, April 1993 (Vol. 11)

'a work that is both a laboratory manual and a treatise on the physics of crystallization ... this is a modern laboratory manual useful for anyone working in this research area ... Its value to the experimentalist lies in the presentation of techniques and, probably more importantly, in the discussions of crystallization theory.'Patricia C. Weber, The Du Pont Merck Pharmaceutical Co. Acta Cryst. (1994)

Synopsis

In the last decade crystallography has emerged as a key tool for understanding the structures of biological macromolecules, yet the crystallization techniques themselves have been perceived as difficult to perform. This book provides a detailed guide to producing crystals of proteins and nucleic acids for diffraction studies. Standard crystallization methods are given, along with procedures for the preparation and handling of macromolecules. Other topics covered include seeding procedures, crystallization in gels, under microgravity and supergravity, crystallization of nucleic acids, nucleic acid-protein complexes and membrane proteins, an introduction to X-ray crystallography and automated processes. Detailed laboratory protocols and the underlying theoretical background are brought together, providing researchers with the conceptual and methodological tools necessary to exploit these techniques.

Edition details

• Publisher: IRL Press
• Publication date: 1992
• Language: English
• ISBN 10: 0199632464
• ISBN 13: 9780199632466
• Binding: Paperback
• Number of pages: 360
• Editor: Ducruix A., Giegé R.