The two volumes of Essential Molecular Biology: A Practical Approach provide clear theoretical and practical guidance to the fundamental techniques of recombinant DNA analysis. Although of particular value to research workers entering the field for the first time, the protocols described will also be of interest to experienced researchers.
Volume I: concentrates on the preparation, purification and handling of nucleic acids, and includes chapters on microbiological procedures and the construction and use of recombinant DNA molecules. Each chapter is written in an accessible style by an acknowledged expert in that field with the emphasis on providing sound technical instruction, in combination with the necessary theoretical background and useful troubleshooting guidance. This book thus brings powerful techniques at the forefront of molecular biology research within reach of any researcher. Together, the two volumes provide a comprehensive molecular biology manual tailored specially for the novice.
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'presents essential techniques of molecular biology in a practical and pragmatic manner ... The value of this volume is that it is succinct and direct.' Trends in Pharmacological Sciences, Volume 12 No. 11
'This is an extremely useful volume. not only for the newcomer but also to keep in the laboratory to double-check the practical points for carrying out successful molecular biological techniques.' Cell Biology International Reports, Vol.15, No.10, 1991
'Together with volume 2 the book provides a comprehensive manual for the novice in the field of molecular biology.' ASLIB Booklist, Vol.56, No.10. October 1991
'The present volume has maintained the high standards of the practical approach series. Contributors to this volume are experts in their own fields and are very familiar with the practical know-how for each experiment. The Editor has put together a well balanced collection of experiments describing the current state of knowledge of recombinant DNA technology. This volume will I am sure serve as a very valuable basic guide to introduce recombinant DNA technology in any biological laboratory.'Saad Tayyab, Biochemical Education 20(3) 1992
Like most scientific disciplines, the techniques for studying genes and DNA are complicated and difficult for the novice to understand. This book provides an introduction to practical molecular biology and is aimed specifically at students and other research workers who have no prior experience of the subject. In this part of the volume the fundamental techniques for handling and cloning DNA are described, with the emphasis on sound technical instruction, together with theoretical background and trouble-shooting guidance. The aim is to give the reader a sufficiently strong knowledge of these general techniques to allow the procedures to be used with confidence in specific research applications.
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