Receptor Biochemistry: A Practical Approach (Practical Approach Series): 65 - Softcover

Softcover

ISBN 10: 0199630933 ISBN 13: 9780199630936

Publisher: Oxford University Press, USA, 1990

Synopsis

The acquisition of three-dimensional structural information about receptors, particularly through the application of crystallographic and other physical techniques requires the purification and handling of significant amounts of receptor proteins. This book focuses particularly on strategies which have proven successful in the purification of a number of receptors of pharmacological importance. Included are examples of C-protein-coupled receptors, oligomeric receptors which incorporate an integral ion channel, and receptor tyrosine kinases. Specific aspects include the choice of detergents for solubilization, the synthesis and utilization of specific affinity gels, and peptide mapping, among other key topics. There is also an introduction to structure prediction from receptor sequences, and the high-level expression of cloned receptor genes. The book draws on the experience and insight of a number of scientists who have been at the forefront of progress in this difficult and experimentally demanding field.

About this title

Review

'obviously useful ... Cross referencing is good and the book has been well organized so that generalisations appear convincing and principles emerge. In this sense, it is a very well-structured receipe book.' Dr. K.E. Mogensen, Institut de Recharches Scientifiques sur le Cancer, Villejuif, France, Methods in Molecular and Cellular Biology

'Cross referencing is good and the book has been well organized so that generalisations appear convincing and principles emerge ... it is a very well-structured recipe book.'Methods in Molecular and Cellular Biology

'This volume will be a valuable resource of proven protocols, expert guidance, and insightful comment.'Anticancer Research 12: (1992)

`This volume will be a valuable resource of proven protocols, expert guidance, and insightful coment, drawn from the experience of researchers at the forefront of this difficult and experimentally demanding field.'Anticancer Research

Edition details

• Publisher: Oxford University Press, USA
• Publication date: 1990
• Language: English
• ISBN 10: 0199630933
• ISBN 13: 9780199630936
• Binding: Paperback
• Number of pages: 346
• Editor: Hulme E. C.