Introduction to Immunocytochemistry: v. 11 (Royal Microscopical Society Microscopy Handbooks) - Softcover

Polak, Julia M.; Noorden, Susan Van

Softcover

ISBN 10: 0198564155 ISBN 13: 9780198564157

Publisher: Bios Scientific Publishers Ltd, 1987

Synopsis

This work contains a concise exposition of the different immunocytochemical methods for identifying tissue constituents, discussing ways of preparing tissues according to the antigen to be localized and the most commonly used methods. The text is illustrated with explanatory diagrams and photomicrographs. An appendix contains step-by-step instructions for many of the basic techniques at light and electron microscopical levels. Other sections deal with sources of error and nonspecificity, and techniques for recording results photographically. For this revised edition, the authors have updated the text in light of the latest developments and added 20 new references to the literature.

About this title

Review

"The small size of this book is misleading. It has a wealth of theoretical and practical information. ...This book is a classic of immunhostochemistry, with the necessary information to help novices and seasoned individuals explore specific areas. The information presented is clear... This affordable book should be in every laboratory performing immunohistochemistry as well as on the bookshelf of researchers and diagnosticians who rely on this technique.
Dr. Jose Ramos-Vara
Department of Veterinary Pathobiology, Purdue University
."
#41:5
-Veterinary Pathology, 2004
..."Highly recommended for academic and special libraries. The informative content and high level of readability makes this a desirable item for collections with relevant programs."
."
-E-Streams, October 2004

Synopsis

The use of immunocytochemical methods, introduced in the 1950s, has gradually expanded. They are now among the most widely used means of identifying tissue constituents, whether in biological research or for pathological diagnosis. The technique exploits the highly specific reaction between antigen and antibody, by using labelled immune reagents to localize antigens in situ . Ways of preparing tissues for the different methods and according to the antigen to be localized are discussed. The most common methods are described in detail with explanatory diagrams and photomicrographs. More complicated methods are touched on, with a full reference list being provided. Sources of error and non-specificity are pointed out. One chapter deals with recording results photographically. An appendix contains step-by-step practical instructions for many of the basic techniques at light and electron microscopical levels. A newcomer to the field will find enough information to understand the principles of the subject and to be able to produce acceptable results.

For this revised edition, the authors have updated the text in the light of the latest developments, and added 20 new references to the literature and a new colour illustration.

Edition details

• Publisher: Bios Scientific Publishers Ltd
• Publication date: 1987
• Language: English
• ISBN 10: 0198564155
• ISBN 13: 9780198564157
• Binding: Paperback
• Edition number: 2
• Number of pages: 72