Fluorescence Microscopy (Royal Microscopy Society Handbooks) - Softcover

Ploem, J. S.; Tanke, H. J.

Softcover

ISBN 10: 0198564082 ISBN 13: 9780198564089

Publisher: Garland Science, 2004

Synopsis

This handbook provides a thorough and detailed introduction to fluorescence microscopy by exploring three main areas of the field. First discussed are the physical principles of the phenomenon of fluorescence and fluorescence microscopy, including transmitted versus incident illumination. Next is a detailed description of various microscope parts, including lenses, filters, and light sources, and a discussion of the main applications of fluorescence microscopy, including immunofluorescence, microflurometry, and photomicrography. The final section enables the reader to solve the most common problems in fluorescence microscopy. Each chapter includes an up-to-date list of review articles and books for further reading, and there are illustrations throughout the text of typical microscopes and optical components. Biologists, students, and researchers of fluorescence microscopy will value this clear and indispensable guide to the field.

About this title

Synopsis

This handbook covers three main areas. Firstly it deals with the physical principles of the phenomenon of fluorescence and fluorescence microscopy (including transmitted versus incident illumination), and gives a detailed description of the various parts of the microscope: lenses, filters, and light sources. It goes on to discuss the main applications of fluorescence microscopy, for example immunofluorescence, microfluoremetry (including absorbance and fluorescence photometry), and fluorescence photomicrography. The final chapter, on trouble-shooting, will enable the reader to solve the most common problems in fluorescence microscopy. Each chapter is followed by an up-to-date list of references to review articles, books, and specialist publications. Throughout the book, the authors have taken care to emphasize the general aspects of fluorescence microscopy as well as illustrating their description with examples of typical microscopes and optical components. Readership: cell biologists, clinicians, researchers and technicians, and undergraduates in the life and medical sciences interested in obtaining a thorough grounding in fluorescence microscopy.

Edition details

• Publisher: Garland Science
• Publication date: 2004
• Language: English
• ISBN 10: 0198564082
• ISBN 13: 9780198564089
• Binding: Paperback
• Edition number: 1
• Number of pages: 64