This account of ways in which biological problems may be solved with the aid of histochemical methods contains information on a wide variety of specific staining techniques, offers a technical background to formal educational coursework in the biological sciences, and provides the scientific principles for staining biological specimens. The book clarifies types of information deriving from histochemical investigations, eg what is a particular object in biological terms, and where in the cell (or creature) it is found. The modes of preparation needed for particular specimen types are reviewed, as are methods of stabilization, and formation of optically thin layers. The general physical-chemical and biochemical background for histochemical staining is outlined, and from these foundations a number of important topics are addressed.
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