A Practical Guide to the Study of Calcium in Living Cells: A Practical Guide to the Study of Calcium in Living Cells v. 40 (Methods in Cell Biology): Volume 40 - Hardcover

Hardcover

ISBN 10: 0125641419 ISBN 13: 9780125641418

Publisher: Academic Press, 1994

Synopsis

A Practical Guide to the Study of Calcium in Living Cells describes popular techniques along with helpful do's and don't's and computer programs. The volume enables investigators to evaluate confocal images, use the latest dyes, and design Calcium buffers appropriate to their research needs. This book is designed for laboratory use by graduate students, technicians, and researchers in many disciplines, ranging from molecular to cellular levels of investigation.

• Describes techniques for detection of [Ca2+]I: Ca2+ - sensitive microelectrodes
• Fluorescent dyes
• Luminescent proteins
• Includes techniques for perturbing intracellular Ca2+
• Covers detailed methodology plus problems and pitfalls of each technique
• Contains a practical guide to preparing Ca2+ buffers with an easy-to-use computer program
• Color plates illustrate techniques such as
• Confocal ratio-imaging
• Use of aequorin

About this title

About the Author

University of California, Santa Barbara, USA

Edition details

• Publisher: Academic Press
• Publication date: 1994
• Language: English
• ISBN 10: 0125641419
• ISBN 13: 9780125641418
• Binding: Hardcover
• Number of pages: 384
• Editor: Nuccitelli Richard