Cell Biological Applications of Confocal Microscopy (Volume 38) (Methods in Cell Biology, Volume 38) - Hardcover

Hardcover

ISBN 10: 0125641389 ISBN 13: 9780125641388

Publisher: Academic Press Inc, 1993

Synopsis

Cell Biological Applications of Confocal Microscopy instructs researchers on methods of preparing biological material for confocal microscopy. Individual chapters describe the technical problems of imaging structures in thick specimens and useful techniques such as multi-color dye visualization. The book provides specific examples of applications of confocal microscopy to cell biological problems. This volume is an essential guide for students and scientists incell biology, neuroscience, and many other areas of biological and biomedical research, as well as research directors and technical staff of microscopy and imaging facilities.

About this title

Review

Praise for the Series: "The series is invaluable for workers at all levels of cell biology." --Nature

Praise for the first edition: "The diverse applications presented in this book reflect the creativity and technical skills of the many contributors while emphasizing the power and promise of CM." --Trends in Cell Biology

From the Back Cover

Cell Biological Applications of Confocal Microscopy is a comprehensive introduction to confocal microscopy for cell biologists. It contains integrated and up-to-date coverage on the various techniques and uses of this valuable research tool. Techniques are described completely and methods are made accessible and understandable, enabling the researcher to study and develop a hands-on approach to imaging. Chapters cover a variety of topics ranging from confocal microscope theory to applications for studies in cell biology. Fundamental topics include discussion on the role of coverglass thickness in 3-D imaging, and selecting a suitable pixel size. More advanced topics include the use of the microscope in ratiometric measurements with physiological dyes, the application of fast video rate microscopes, and the two-photon microscope and its advantages over traditional confocal microscopes. Chapters of interest to developmental and molecular biologists describe the properties of imaging species of animals, the Drosophilia embryo, and the single cell organism, Tetrahymena.

Key Features
* Includes detailed protocols accessible to new users
* Contains an overview of available confocal microscopes
* Details how to set up and run a "Confocal Microscope Core Facility"
* Contains over 170 figures|Cell Biological Applications of Confocal Microscopy is a comprehensive introduction to confocal microscopy for cell biologists. It contains integrated and up-to-date coverage on the various techniques and uses of this valuable research tool. Techniques are described completely and methods are made accessible and understandable, enabling the researcher to study and develop a hands-on approach to imaging. Chapters cover a variety of topics ranging from confocal microscope theory to applications for studies in cell biology. Fundamental topics include discussion on the role of coverglass thickness in 3-D imaging, and selecting a suitable pixel size. More advanced topics include the use of the microscope in ratiometric measurements with physiological dyes, the application of fast video rate microscopes, and the two-photon microscope and its advantages over traditional confocal microscopes. Chapters of interest to developmental and molecular biologists describe the properties of imaging species of animals, the Drosophilia embryo, and the single cell organism, Tetrahymena.

Key Features
* Includes detailed protocols accessible to new users
* Contains an overview of available confocal microscopes
* Details how to set up and run a "Confocal Microscope Core Facility"
* Contains over 170 figures

Edition details

• Publisher: Academic Press Inc
• Publication date: 1993
• Language: English
• ISBN 10: 0125641389
• ISBN 13: 9780125641388
• Binding: Hardcover
• Number of pages: 380