A Practical Guide to Protein and Peptide Purification for Microsequencing - Softcover

Softcover

ISBN 10: 0124802826 ISBN 13: 9780124802827

Publisher: Academic Press, 1993

Synopsis

Why a Second Edition? The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry.

About this title

From the Back Cover

Why a Second Edition?
The second edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry.
@introbul:Highlights of the Second Edition:
@bul:* Mass spectrophotometric characterization of proteins and peptides
* N-terminal sequencing of proteins with blocked N termini
* Internal amino acid sequence analysis after protease digestion in-gel and on-blot
* Improved microscale peptide purification methods
* Computer analysis of protein sequences
* New protocols tested and refined through everyday use in authors' laboratories
* Updated reference chapter covering all aspects of protein microsequencing

Edition details

• Publisher: Academic Press
• Publication date: 1993
• Language: English
• ISBN 10: 0124802826
• ISBN 13: 9780124802827
• Binding: Plastic Comb
• Edition number: 2
• Number of pages: 204
• Editor: Matsudaira Paul T.