Cellular Connectomics: Reconstruction of Complete Neural Wiring Diagrams

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9780124103955: Cellular Connectomics: Reconstruction of Complete Neural Wiring Diagrams

The field of cellular connectomics is technically challenging and highly multi-disciplinary, creating need for a connectomics methods volume suitable for both researchers and students aiming to gain familiarity with the field. Cellular Connectomics offers just that: an accessible, practical and comprehensive introduction to the fundamental principles and practices of cellular connectomics. With the bulk of the volume written by two pioneers in the field, plus 3 chapters authored by opinion leaders offering future predictions, the volume offers readers both consistent voice/uniform structure and cutting edge insights. Focusing on "cellular" connectomics with the goal of reconstructing neuronal wiring diagrams at the level of single neurons/synapses, the volume provides a conceptual overview of the goals and challenges of this methodology. * The first volume to offer a step-by-step introduction to cellular connectomics suitable for both researchers and students.* Coverage includes: tissue preparation, sectioning and imaging techniques, approaches for data reconstruction, circuit analysis, and an explanation of successful connectomic reconstructions to date.* Offers balanced discussion what does and does not work, as well as of the current debates about whether a connectomic era is going to replace decades of functional data collection.

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About the Author:

Dr. Helmstaedter has pioneered large-scale circuit reconstruction using crowd-sourcing and machine learning. His laboratory spearheads large-scale crowd sourcing and online annotation approaches for connectomics, with a focus on the connectomic reconstruction of the cerebral cortex. Dr. Briggman brings specific expertise in the tissue preparation and imaging methods. He is heading one of the few laboratories in the world that are able to produce and analyze connectomic datasets.

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