Digital Microscopy (Volume 114) (Methods in Cell Biology, Volume 114) - Hardcover

 
9780124077614: Digital Microscopy (Volume 114) (Methods in Cell Biology, Volume 114)
Hardcover
ISBN 10: 0124077617 ISBN 13: 9780124077614
Publisher: Academic Press, 2013

Synopsis

The previous edition of this book marked the shift in technology from video to digital camera use with microscope use in biological science. This new edition presents some of the optical fundamentals needed to provide a quality image to the digital camera. Specifically, it covers the fundamental geometric optics of finite- and infinity-corrected microscopes, develops the concepts of physical optics and Abbe’s theory of image formation, presents the principles of Kohler illumination, and finally reviews the fundamentals of fluorescence and fluorescence microscopy. The second group of chapters deals with digital and video fundamentals: how digital and video cameras work, how to coordinate cameras with microscopes, how to deal with digital data, the fundamentals of image processing, and low light level cameras. The third group of chapters address some specialized areas of microscopy that allow sophisticated measurements of events in living cells that are below the optical limits of resolution.

  • Expands coverage to include discussion of confocal microscopy not found in the previous edition
  • Includes "traps and pitfalls" as well as laboratory exercises to help illustrate methods

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Review

Provides the essential tools and methods for working with digital microscopy as a technique for quantitative biological research.

"About this title" may belong to another edition of this title.

Publisher
Academic Press
Publication date
2013
Language
English
ISBN 10 
0124077617
ISBN 13 
9780124077614
Binding
Hardcover
Edition number
4
Number of pages
672
Editor
Sluder Greenfield, Wolf David E.