The Zebrafish: Cellular and Developmental Biology, Part A, Volume 133, Third Edition (Methods in Cell Biology)

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9780123848925: The Zebrafish: Cellular and Developmental Biology, Part A, Volume 133, Third Edition (Methods in Cell Biology)

This volume of Methods in Cell Biology, the first of 3 parts on the subject of zebrafish, provides a comprehensive compendia of laboratory protocols and reviews covering all the new methods developed since 2004. This first volume provides state-of-the-art descriptions of novel cellular imaging technologies and methods for culture of zebrafish stem cells, summarizes protocols for analyzingthe development of major organ systems including the central nervous system (CNS), and introduces the use of the zebrafish as a model system for human diseases.

  • Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls
  • Summarizes the Zebrafish Genome Project

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From the Back Cover:

An underlying principle of developmental biology is that invertebrate species, such as flies or fish, employ essentially the same signaling systems to establish the body plan as vertebrates. While the mouse may be the animal model of choice for studies of vertebrate development, the fact that most of mouse embryonic development occurs in the mother's uterus makes it quite difficult to study. The zebrafish, which produces large numbers of embyros that develop outside the mother's body, has emerged as the preeminent genetic system for developmental analysis.

This volume of Methods in Cell Biology, the second of two parts on the subject of zebrafish, provides a comprehensive compendium of laboratory protocols and reviews covering all the new methods developed since 1999.

About the Author:

Professor of Biochemistry and Marine Biology at Northeastern University, promoted 1996. Joined Northeastern faculty in 1987. Previously a faculty member in Dept. of Biochemistry at the University of Mississippi Medical Center, 1983-1987.Principal Investigator in the U.S. Antarctic Program since 1984. Twelve field seasons "on the ice" since 1981. Research conducted at Palmer Station, Antarctica, and McMurdo Station, Antarctica.Research areas: Biochemical, cellular, and physiological adaptation to low and high temperatures. Structure and function of cytoplasmic microtubules and microtubule-dependent motors from cold-adapted Antarctic fishes. Regulation of tubulin and globin gene expression in zebrafish and Antarctic fishes. Role of microtubules in morphogenesis of the zebrafish embryo. Developmental hemapoiesis in zebrafish and Antarctic fishes. UV-induced DNA damage and repair in Antarctic marine organisms.

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