Stem Cell Culture: Methods in Cell Biology: Volume 86 - Hardcover

Mather

Hardcover

ISBN 10: 0123738768 ISBN 13: 9780123738769

Publisher: Academic Press, 2008

Synopsis

The purpose of Stem Cell Culture is to provide a comprehensive resource for researchers in the fields of embyonic, fetal and adult stem cell biology to find methods for the purification, culture, and differentitation of these cell types, with the main emphasis on the maintenance of the stem cell phenotype in vitro. This volume will be the first to broadly cover multiple types of stem cell culture from different ages, organs and species. Authors will focus on the practical do's and don'ts of isolating and culturing these cell types, and feel free to use illustrative data or diagrams whereever this improves the comprehension of the reader. This should allow the reader to compare and contrast techniques and make this a standard reference for those in the field, or desiring to start stem cell culture.

• Describes techniques in stem cell research
• Delineates critical steps and potential pitfalls for each method
• Covers specific procedures in dealing with Human Embryonic Stem Cells

About this title

Review

This volume will gather together the breadth of current knowledge on the culture of human, and non-human, stem cells looking at both totipotent and puripotent stem cells from embryonic, fetal and adult tissue sources.

Synopsis

Interest in the therapeutic value of embryonic, fetal and adult stem cell types is rapidly expanding throughout the scientific community. The first half of this century should see an explosion of therapeutic applications of stem cells which will grow from the cells and techniques described in this book. "Stem Cell Culture" provides a comprehensive resource for researchers in the fields of embryonic, fetal and adult stem cell biology to find methods for the purification, culture, and differentiation of these cell types, with the main emphasis on the maintenance of the stem cell phenotype in vitro.This volume will be the first to broadly cover multiple types of stem cell culture from different ages, organs and species. The chapters focus on the practical do's and don'ts of isolating and culturing these cell types, and use illustrative data or diagrams that allow the reader to confidently apply techniques and make this a standard reference. It compares and contrasts a broad range of species and stem cell types. It describes techniques in stem cell research, and goes beyond just protocols by presenting methods within perspective of recent literature reviews.

It delineates critical steps and potential pitfalls for each method, provided by at-the-bench authors for expert information. It covers specific procedures in dealing with Human Embryonic Stem Cells, but also emphasizes other sources of potentially therapeutic stem cells such as amniotic fluid, adult muscle and adipose tissue, and umbilical cord.

Edition details

• Publisher: Academic Press
• Publication date: 2008
• Language: English
• ISBN 10: 0123738768
• ISBN 13: 9780123738769
• Binding: Hardcover
• Number of pages: 250